Ø Dimethylsulfoxide
(DMSO) is known for its
(i) capacity to dissolve compounds with a wide range of chemical properties
(ii) it has low volatility
(iii) relatively low toxicity to both tissue culture and technicians
(iv) it has good miscibility with water, and limited deleterious effects at low concentration upon bioassays.
(i) capacity to dissolve compounds with a wide range of chemical properties
(ii) it has low volatility
(iii) relatively low toxicity to both tissue culture and technicians
(iv) it has good miscibility with water, and limited deleterious effects at low concentration upon bioassays.
Ø Though DMSO has become the de facto solvent of choice for compound
solubilization for HTS, DMSO has its shortcomings
(i) DMSO can act as oxidizing agent. You can oxidize suitable compounds by leaving them in DMSO open to air
(ii) DMSO is highly hygroscopic.
(iii) lipophilic compounds might not have appropriate solubility in DMSO (sometimes completely insoluble).
(i) DMSO can act as oxidizing agent. You can oxidize suitable compounds by leaving them in DMSO open to air
(ii) DMSO is highly hygroscopic.
(iii) lipophilic compounds might not have appropriate solubility in DMSO (sometimes completely insoluble).
Ø ADMET data is obtained for compounds as methanol stocks and
enzyme assay most often are performed with DMSO stocks. The reason for such solvent selection depends
on what read-out you have.
ADMET
(fluorescence read out) – therefore, DMSO is not a compatible solvent.
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